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alp  (R&D Systems)


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    Structured Review

    R&D Systems alp
    Alp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+alkaline+phosphatase/Human+Alkaline+Phosphatase%2FALPL+Antibody/pm41961374-109-25-26
    Average 94 stars, based on 9 article reviews
    alp - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    FACS:

    Article Title: Human motor units in microfluidic devices are impaired by FUS mutations and improved by HDAC6 inhibition
    Article Snippet: .. After 14 days, cells were fluorescent activated cell (FACS)-sorted for human alkaline phosphatase (R&D systems, Minneapolis, MN, USA, Cat N° MAB1448) and expanded further in T75 flasks (Sigma, Cat N° CLS3276) on collagen from calf skin (Cat N° 17104019) in growth medium. .. Cells were cryopreserved in knockout serum replacement (Cat N° 10828-028) with 10% DMSO (Sigma, Cat N° D2650100ML), passaged, or seeded in devices when reaching 70% confluence.

    Article Title: Human motor units in microfluidic devices are impaired by FUS mutations and improved by HDAC6 inhibition
    Article Snippet: .. After 14 days, cells were fluorescent activated cell (FACS)-sorted for human alkaline phosphatase (R&D systems, Minneapolis, MN, USA, Cat N° MAB1448) and expanded further in T75 flasks (Sigma, Cat N° CLS3276) on collagen from calf skin (Cat N° 17104019) in growth medium. .. Cells were cryopreserved in knockout serum replacement (Cat N° 10828-028) with 10% DMSO (Sigma, Cat N° D2650100ML), passaged, or seeded in devices when reaching 70% confluence.

    Article Title: Human motor units in microfluidic devices are impaired by FUS mutations and improved by HDAC6 inhibition
    Article Snippet: .. After 14 days, cells were fluorescent activated cell (FACS)-sorted for human alkaline phosphatase (R&D systems, Minneapolis, MN, USA, Cat N° MAB1448) and expanded further in T75 flasks (Sigma, Cat N° CLS3276) on collagen from calf skin (Cat N° 17104019) in growth medium. .. Cells were cryopreserved in knockout serum replacement (Cat N° 10828-028) with 10% DMSO (Sigma, Cat N° D2650-100ML), passaged, or seeded in devices when reaching 70% confluence.



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    Image Search Results


    Biomarkers. Fig. 1 depicts all assessed biomarker concentrations. The total hip, femoral neck, or lumbar spine (Lower) group is depicted in black filled circles, while the control (Con) group is in clear squares. A, P1NP (pg/mL); B, alkaline phosphatase (ALP) (pg/mL); C, tartrate-resistant acid phosphatase 5b (TRAP5b) (U/L); D, sclerostin (pmol/L); E, β C-terminal cross-linked telopeptide of type I collagen (βCTX) (pmol/L); F, testosterone (ng/mL); G, estradiol (pg/mL); H, parathyroid hormone (PTH) (pg/mL); I, insulin-like growth factor-1 (IGF-1) (ng/mL); J, insulin-like growth factor-1 binding protein 5 (IGFBP-5) (ng/mL).

    Journal: Journal of the Endocrine Society

    Article Title: Subclinically low BMD in young men is associated with compromised bone microarchitecture and lower lean mass

    doi: 10.1210/jendso/bvag003

    Figure Lengend Snippet: Biomarkers. Fig. 1 depicts all assessed biomarker concentrations. The total hip, femoral neck, or lumbar spine (Lower) group is depicted in black filled circles, while the control (Con) group is in clear squares. A, P1NP (pg/mL); B, alkaline phosphatase (ALP) (pg/mL); C, tartrate-resistant acid phosphatase 5b (TRAP5b) (U/L); D, sclerostin (pmol/L); E, β C-terminal cross-linked telopeptide of type I collagen (βCTX) (pmol/L); F, testosterone (ng/mL); G, estradiol (pg/mL); H, parathyroid hormone (PTH) (pg/mL); I, insulin-like growth factor-1 (IGF-1) (ng/mL); J, insulin-like growth factor-1 binding protein 5 (IGFBP-5) (ng/mL).

    Article Snippet: Commercially available enzyme-linked immunosorbent assays were used to determine circulating concentrations of serum procollagen type I N-terminal propeptide (PINP; Novus catalog No. NBP2-76465, RRID:AB_3720306, 1:200 dilution, CV 5.3%, sensitivity 9.4 pg/mL), alkaline phosphatase (ALP; Novus catalog No. NBP2-68197, RRID:AB_3720307, CV 6.0%, sensitivity 46.9 pg/mL), tartrate-resistant acid phosphatase 5b (TRAP5b; Immunodiagnostic Systems catalog No. SB-TR201A, RRID:AB_3095995, CV 4.7%, sensitivity 0.4 U/L), sclerostin (Biomedica catalog No. BI-20492, RRID:AB_2894889, CV 6.4%, sensitivity 3.2 pmol/L), testosterone (Alpco Diagnostics catalog No. 11-TESHU-E01, RRID:AB_3720308, CV 4.2%, sensitivity 0.02 ng/mL), estradiol (Alpco Diagnostics catalog No. 11-ESTHU-E01, RRID:AB_2756385, CV 7.6%, sensitivity 10 pg/mL), insulin like growth factor-1 (IGF-1; Alpco Diagnostics catalog No. 22-IGFHU-E01, RRID:AB_2923276, 1:20 dilution, average CV 3.3%, assay sensitivity 9.4 pg/mL), IGF binding protein-5 (IGFBP-5; R and D Systems catalog No. DY875, RRID:AB_3720311, 3× dilution, CV 2.7%), parathyroid hormone (PTH; (Alpco Diagnostics catalog No. 21-IPTHU-E01, RRID:AB_2943035), CV 7.3%, sensitivity 1.6 pg/mL), and plasma β C-terminal cross-linked telopeptide of type I collagen (βCTX; Immunodiagnostic Systems catalog No. IS-3000N, RRID:AB_3720313, CV 6.2%, sensitivity 3.2 pmol/L).

    Techniques: Biomarker Discovery, Control, Binding Assay

    Cell culture. Fig. 2 depicts the results of the cell culture experiment over 10 days. The total hip, femoral neck, or lumbar spine (Lower) group is depicted in black filled circles, while the control (Con) group is in clear squares. A, Alizarin red staining; B, alkaline phosphatase staining.

    Journal: Journal of the Endocrine Society

    Article Title: Subclinically low BMD in young men is associated with compromised bone microarchitecture and lower lean mass

    doi: 10.1210/jendso/bvag003

    Figure Lengend Snippet: Cell culture. Fig. 2 depicts the results of the cell culture experiment over 10 days. The total hip, femoral neck, or lumbar spine (Lower) group is depicted in black filled circles, while the control (Con) group is in clear squares. A, Alizarin red staining; B, alkaline phosphatase staining.

    Article Snippet: Commercially available enzyme-linked immunosorbent assays were used to determine circulating concentrations of serum procollagen type I N-terminal propeptide (PINP; Novus catalog No. NBP2-76465, RRID:AB_3720306, 1:200 dilution, CV 5.3%, sensitivity 9.4 pg/mL), alkaline phosphatase (ALP; Novus catalog No. NBP2-68197, RRID:AB_3720307, CV 6.0%, sensitivity 46.9 pg/mL), tartrate-resistant acid phosphatase 5b (TRAP5b; Immunodiagnostic Systems catalog No. SB-TR201A, RRID:AB_3095995, CV 4.7%, sensitivity 0.4 U/L), sclerostin (Biomedica catalog No. BI-20492, RRID:AB_2894889, CV 6.4%, sensitivity 3.2 pmol/L), testosterone (Alpco Diagnostics catalog No. 11-TESHU-E01, RRID:AB_3720308, CV 4.2%, sensitivity 0.02 ng/mL), estradiol (Alpco Diagnostics catalog No. 11-ESTHU-E01, RRID:AB_2756385, CV 7.6%, sensitivity 10 pg/mL), insulin like growth factor-1 (IGF-1; Alpco Diagnostics catalog No. 22-IGFHU-E01, RRID:AB_2923276, 1:20 dilution, average CV 3.3%, assay sensitivity 9.4 pg/mL), IGF binding protein-5 (IGFBP-5; R and D Systems catalog No. DY875, RRID:AB_3720311, 3× dilution, CV 2.7%), parathyroid hormone (PTH; (Alpco Diagnostics catalog No. 21-IPTHU-E01, RRID:AB_2943035), CV 7.3%, sensitivity 1.6 pg/mL), and plasma β C-terminal cross-linked telopeptide of type I collagen (βCTX; Immunodiagnostic Systems catalog No. IS-3000N, RRID:AB_3720313, CV 6.2%, sensitivity 3.2 pmol/L).

    Techniques: Cell Culture, Control, Staining